Using a quadruplet codon to expand the genetic code of an animal

Zhiyan Xi, Lloyd Davis, Kieran Baxter, Ailish Tynan, Angeliki Goutou, and Sebastian Greiss

Nucleic Acids Research, gkab1168, doi:10.1093/nar/gkab1168

A team of investigators describe the first example of the development and use of a novel ‘quadruplet codon’ system for the incorporation of a non-canonical amino acids at specific positions with a protein encoded by a multicellular organism (the nematode worm C. elegans). This approach offers the prospect of incorporating multiple altered amino acids concurrently in complex systems such as multicellular eukaryotes, where genome recoding, unlike in bacteria, is not an option.

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