Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome

Keisuke Oki, Mariko Nagata, Takeshi Yamagami, Tomoyuki Numata, Sonoko Ishino, Takuji Oyama, and Yoshizumi Ishino

Nucleic Acids Research, gkab799, doi:10.1093/nar/gkab799

Genomic DNA replication requires replisome assembly, in which parental double-stranded DNA (dsDNA) is unwound by helicases into single-stranded DNA (ssDNA). DNA polymerases then synthesize nascent strands. The replisome assembly mechanism in archaea is still poorly understood. In particular, the mechanism by which archaeal replicative polymerases are coordinated with corresponding helicase factors remains unclear. Here, the investigators demonstrate the molecular basis for archaeal replisome assembly, including an atomic structure of the DP1N–Gins51C–GAN ternary complex, which is believed to act as the replisome core in the model archaeon T. kodakarensis. A structural comparison of comparable regions within the archaeal and yeast replisomes revealed that the structural basis for the interaction between their polymerases and the replicative helicase is conserved in two domains of life. These findings provide insight into the archaeal replisome assembly and helicase activation.

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