Construction of a highly error-prone DNA polymerase for developing organelle mutation systems

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Junwei Ji, and Anil Day

Nucleic Acids Research, 2020, 48 (21): 11868–11879, https://doi.org/10.1093/nar/gkaa929

The study of how organellar genomes (found in chloroplasts and mitochondria) are maintained, and methods for study of that process is less well understood than corresponding processes in nuclear genomes, particularly outside of the animal kingdom. In this study, investigators construct and characterize the first highly error prone version of an organellar DNA polymerase to study the mechanism that govern organellar genome maintenance in non-animal systems.

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