IncC conjugative plasmids and SXT/R391 elements repair double-strand breaks caused by CRISPR–Cas during conjugation

David Roy, Kevin T Huguet, Frédéric Grenier, and Vincent Burrus

Nucleic Acids Research, gkaa518,

The spread of antibiotic resistance by mobile genetic elements is a serious world-wide public health issue. Therefore, a clear understanding of the mechanisms by which these vectors can spread and persist in a bacterial population is crucial to develop new control strategies. In this study, the authors describe several discoveries regarding how the mobile genetic elements that contain antibiotic resistance genes evade destruction by bacterial defense systems (such as CRISPR and restriction enzymes) that normally block the import of new genetic material.

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