Biphasic unbinding of a metalloregulator from DNA for transcription (de)repression in Live Bacteria

Won Jung, Kushal Sengupta, Brian M Wendel, John D Helmann, and Peng Chen

Nucleic Acids Res (2020) 48(5): 2199–2208, doi:10.1093/nar/gkaa056

By combining single-molecule tracking, single-cell protein quantitation, quantitative kinetic analysis, and genetic engineering, the authors have made novel discoveries on how the behavior of a metal-responsive transcription regulator in live bacterial cells challenges two long-standing mechanistic paradigms in regulator-DNA interactions.

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